LoTemp^™ PCR^*  with HiFi^® DNA polymerase 

unloading the power of automated Sanger sequencing

in DNA testing for Pap cytology specimens

Perform DNA sequencing for HPV genotyping beyond a reasonable doubt -  BMC Clin Path 2009, 9:3 (22 May 2009).   

No-false positive Lyme disease DNA testing;

Validate the DNA of Neisseria gonorrhoeae opa genes beyond a reasonable doubt;

Validate the DNA of Chlamydia trachomatis cryptic plasmid beyond a reasonable doubt;

Using the GenBank database as the standard for validating sequence algorithms;

On 5% of a liquid-based Pap cytology specimen without need of DNA extraction.

These are cutting-edge molecular tests developed by pathologists/ cytotechnologists for pathologists/cytotechnologists to serve gynecologists and their patients at sustainable cost.  

Application of LoTemp® PCR/DNA sequencing recent publications:

1. Increased Sensitivity and Specificity of Borrelia burgdorferi 16S Ribosomal DNA Detection. Am J Clin Pathol. 2010 Apr;133(4):569-76.

2. Signature sequence validation of human papillomavirus type 16 (HPV-16) in clinical specimens. J Clin Pathol. 2010 Mar;63(3):235-9.

Visit www.hpvtyping.com - Milford Medical Laboratory will work with you for technology transfer, or perform the tests for you at sustainable third-party-reimbursed cost. 

* Lee SH, Vigliotti VS, Vigliotti JS, Pappu S, Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. Infect Agent Cancer 2007:2:11.

HiFiDNA.com © 2006-2009